Biotinylation reagent with N-hydroxysuccinimide, and very long (12 PEG) spacer. The NHS group reacts with the e-amino group on the N-terminus of proteins, and other primary amines. A coupling agent such as EDC is required to activate the reaction.

≥96%

Soluble in DMSO

White to off-white solid. May appear sticky.

Store at or below ≤4°C. Solid form is stable at least 2 years from date of receipt, when stored as directed.

Useful for biotinylation of proteins with free primary amine groups.

Application Reference(s):
1. Pipette tip biosensors for bacterial double-stranded DNA using bioluminescence induced by zinc finger luciferase (2017)

Protect from moisture. Store with desiccant.

PEG Product, Biotin

Biotinylation Reagent