The SBE Reporter kit is designed for monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.

SBE reporter, TGFβ reporter, TGFbeta reporter, SMAD reporter , Cell based assay kit, cell-based assay, reporter assay kit

Reporter: SBE luciferase reporter vector + constitutively-expressing Renilla luciferase vector
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector

Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)

assay kit

See data sheet for detailed transfection and assay protocols.

Stable at least 12 months from date of receipt, when stored as directed (-20°C)

• Monitor TGFβ signaling pathway activity.
• Screen for activators or inhibitors of the TGFβ/ SMAD signaling pathway.
• Study effects of RNAi or gene overexpression on the activity of the TGFβ pathway.

Moustakas A et al. (2001) Smad regulation in TGF-beta signal transduction. J.Cell Science. 114(Pt 24): 4359-69.

The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria.

Avoid freeze/thaw cycles

Cell Signaling Pathway

Reporter Assay Kit