Uninfected Human Foreskin Fibroblast cells lysed with Triton X-100.
For research use only.
APPLICATION FOR USE
- ELISA
- Western blot
- Dot blot
- Other Protein-Based Assays
QUALITY CONTROL TESTING INCLUDES
- Sterility
- ELISA
- SDS-PAGE
- Protein Concentration
SHIPPING AND STORAGE:
This product is shipped frozen on dry ice. Store at 70°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
RECOMMENDATIONS:
Before use, if any precipitate is observed, thaw the frozen vial in a waterbath set at 37°C, vortex to mix, centrifuge at 3000 RPM for 5 minutes at 4°C. Aspirate the supernatant carefully and use for the intended assay.
SAFE HANDLING RECOMMENDATIONS:
This biological preparation should be handled in accordance with biosafety guidelines defined in the BMBL, NIH-CDC HHS Publication No. (CDC) 21-1112.
FAQ
How are ABI’s Virus Infected/Uninfected Cell Extracts supplied? All ABI antigens are supplied frozen on dry ice.
How are ABI’s Virus Infected/Uninfected Cell Extracts purified? Purification methodology varies according to the individual product requirements, as do the buffers in which the antigens are frozen. ABI offers a variety of antigens (in different purities) to address individual assay/cost requirements. We recommend that you contact us with your assay specifications so that we may suggest the appropriate form and purity.
How should Virus Infected/Uninfected Cell Extracts be stored? We recommend that all antigens be stored frozen at -70°C or below. Avoid multiple freeze-thaw cycles as this will affect antigen activity. Do not dilute and freeze — make final dilution just prior to use.
How is the protein concentration of ABI’s Virus Infected/Uninfected Cell Extracts determined? A modified BCA protein assay is used with Bovine Serum Albumin (BSA) as the protein standard.
