Herpes Simplex Virus type 2 (G Strain) infected Vero cells lysed with Triton X-100.
For research use only.
APPLICATION FOR USE
- ELISA
- Western blot
- Dot blot
- Other Protein-Based Assays
- Antibody Screening/Titration
QUALITY CONTROL TESTING INCLUDES
- Sterility
- Validation of Inactivation
- ELISA
- Protein Concentration
SHIPPING AND STORAGE:
This product is shipped frozen on dry ice. Store at -70°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
RECOMMENDATIONS:
Before use, if any precipitate is observed, thaw the frozen vial in a waterbath set at 37°C, vortex to mix, centrifuge at 3000 RPM for 5 minutes at 4°C. Aspirate the supernatant carefully and use for the intended assay.
SAFE HANDLING RECOMMENDATIONS:
This inactivated biological preparation should be handled in accordance with biosafety guidelines defined in the BMBL, NIH-CDC HHS Publication No. 93-8395.
FAQ
How are ABI’s Virus Infected/Uninfected Cell Extracts supplied? All ABI antigens are supplied frozen on dry ice.
How are ABI’s Virus Infected/Uninfected Cell Extracts purified? Purification methodology varies according to the individual product requirements, as do the buffers in which the antigens are frozen. We recommend that you contact us with your assay specifications so that we may suggest the appropriate form and purity.
How pure are ABI’s Virus Infected/Uninfected Cell Extracts? ABI offers a variety of antigens (in different purities) to address individual assay/cost requirements. We recommend that you contact us with your assay specifications so that we may suggest the appropriate form and purity.
How should Virus Infected/Uninfected Cell Extracts be stored? We recommend that all antigens be stored frozen at -70°C or below. Avoid multiple freeze-thaw cycles as this will affect antigen activity. Do not dilute and freeze — make final dilution just prior to use.
How is the protein concentration of ABI’s Virus Infected/Uninfected Cell Extracts determined? A modified BCA protein assay is used with Bovine Serum Albumin (BSA) as the protein standard.
