产品名称 | HCMV (AD169 Strain) Quantitated Viral DNA | Advanced Biotechnologies | Your Viral Reagent Company |
产品货号 | 08-925-000 |
产品价格 | 现货询价,电话:010-67529703 |
产品规格 | |
产品品牌 | Advanced Biotechnologies |
产品概述 | |
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Human Cytomegalovirus (AD169 Strain) Quantitated Viral DNA PCR control. Researchers who have viewed this product have also considered HCMV (AD169 Strain) Viral qDNA, catalog #17-925-500. ≥ 2,500,000 copies of HCMV DNA per tube For research use only. APPLICATION FOR USE
QUALITY CONTROL TESTING INCLUDES
SHIPPING AND STORAGE:This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result. RECOMMENDATIONS:Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid. SAFE HANDLING RECOMMENDATIONS:The DNA extraction procedure used has been shown to eliminate the infectivity of most viruses and bacteria; therefore, this product is not considered biohazardous. However, this product is not specifically tested for infectivity and should be handled in accordance with Good Laboratory Practices and any applicable local guidelines. FAQ
How are Quantitated PCR controls supplied? Quantitated PCR controls are supplied at 1-2.5 × 104 copies/µL (with the exception of BK and JC polyomaviruses and PCV2, which are supplied at 1-2.5 × 105 copies/µL). The copy number of Quantitated PCR controls is determined by real-time PCR. How can dilutions of Quantitated PCR controls be stored? ABI does not recommend storing dilutions of these controls under any conditions. All dilutions should be made immediately before use and used promptly. We have observed that dilutions used for standard curves typically lose copy number with time — sometimes as soon as an hour or so after dilution. This is especially common at dilutions less than 100-1000 copies per microliter. REFERENCE ARTICLESAgostini, S., Mancuso, R., Guerini, F., D’Alfonso, S., Agliardi, C., Hernis, A., Zanzottera, M., Barizzone, N., Leone, M., and Caputo, D. et al. (2018). HLA alleles modulate EBV viral load in multiple sclerosis. Journal Of Translational Medicine 16:80. https://doi.org/10.1186/s12967-018-1450-6 Okahara, K., Nagata, N., Shimada, T., Joya, A., Hayashida, T., Gatanaga, H., Oka, S., Sakurai, T., Uemura, N., and Akiyama, J. (2017). Colonic cytomegalovirus detection by mucosal PCR and antiviral therapy in ulcerative colitis. PLOS ONE 12, e0183951. https://doi.org/10.1371/journal.pone.0183951 Jeyakumar, A., Warrington, S., Seybolt, L., and Zabaleta, J. (2014). Incidence of Congenital CMV. Otolaryngology-Head And Neck Surgery 151, 238-238. https://doi.org/10.1177/0194599814541629a319 Atkinson, C., Emery, V., and Griffiths, P. (2014). Development of a novel single tube nested PCR for enhanced detection of cytomegalovirus DNA from dried blood spots. Journal Of Virological Methods 196, 40-44. https://doi.org/10.1016/j.jviromet.2013.10.029 Nikitskaya, E., Lebedeva, A., Ivanova, O., Maryukhnich, E., Shpektor, A., Grivel, J., Margolis, L., and Vasilieva, E. (2016). Cytomegalovirus?Productive Infection Is Associated With Acute Coronary Syndrome. Journal Of The American Heart Association 5, e003759. https://doi.org/10.1161/jaha.116.003759 Amin, M., Stowell, J., Hendley, W., Garcia, P., Schmid, D., Cannon, M., and Dollard, S. (2018). CMV on surfaces in homes with young children: results of PCR and viral culture testing. BMC Infectious Diseases 18:391. https://doi.org/10.1186/s12879-018-3318-z
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