Human recombinant nucleosomes consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A (a.a. 2-130(end)), H2B (a.a. 2-126(end)), H3 (a.a. 2-137(end)), and H4 (a.a. 2-103(end)), expressed in an E. coli expression system. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Each histone protein has an N-terminal His-tag. Total histone MW = 113.8 kDa.

Q7L7L0, Q16778, P68431, P62805

20 mM Tris pH 7.5, 1 mM EDTA, 1 mM DTT and 20% glycerol

Aqueous buffer solution

At least 6 months at –80°C. .

Substrate for histone methyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases for drug discovery and HTS applications.

1. Meagher, R.B., Mussar, K.J., Epigenetics Chromatin 2012 Jul 20;5(1):11.
2. Huang, H., et al., Biosystems 2012 Aug;109(2):214-9.

The concentration of this product is determined by Bradford and represents only the protein concentration.

Avoid freeze/thaw cycles.

Methyltransferase

Recombinant Protein