Three types of arginine methylation exist in mammalian cells: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze ADMA, while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. Type II PRMTs are found to be strongly implicated in diseases like cancer. For example, PRMT5 plays a role in the repression of certain tumor suppressor genes such as RB tumor suppressors while PRMT7 overexpression is observed in breast cancer
| 产品名称 | PRMT5 Protein, Protein arginine methyltransferase 5 |
| 产品货号 | 51045 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 20 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: FreeStyle 293-F cells
MW: 73 kDa (PRMT5), 37.5 kDa (MEP50)
Genbank #: NM_006109 (PRMT5), NM_024102 (MEP50)
Tag(s): N-terminal FLAG-tag (PRMT5), N-terminal His-tag (MEP50)
a.a: 2–end (both)
Background: Description: Complex of human PRMT5 protein(GenBank Accession No. NM_006109), a.a. 2-end, with N-terminal FLAG-tag, MW=73 kDa, and human MEP50 (GenBank Accession No. NM_024102), a.a. 2-end, with N-terminal His tag, MW=37.5 kDa, expressed in FreeStyle 293-F cells.
UniProt O14744
Synonym(s): PRMT5, HRMT1L5, IBP72, JBP1, SKB1, PRMT Protein
Assay Conditions: 50 µl reaction mix (20 mM phosphate buffer pH 7.4, 20 µM S-adenosyl methionine, and 10-100 ng methyltransferase PRMT5) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 80 µg/ml Flag peptide, and 20% glycerol.
Format: Aqueous buffer solution
Storage / Stability: At least 6 months at -80°C.
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Zhao Q. et al., J. Nat. Struct. Mol. Biol. 16 (3), 304-311 (2009). Application Reference(s): Warning(s): Avoid freeze/thaw cycles
Scientific Category: Methyltransferase
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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