Human JHDM1D, also known as Lysine-specific demethylase 7A (KDM7A) and KIAA1718, GenBank Accession No. NM_030647 a.a. 2-488 with an N-terminal FLAG-tag, MW=56 kDa, expressed in a baculovirus-infected Sf9 cell expression system.

A0JNV9

Lysine-specific demethylase 7A, KDM7A, KIAA1718

0.2 pmole/min/μg

Assay Conditions: 10 μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 μM alpha-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 μM biotinylated peptide substrate, and JHDM1D (8 – 40ng) added to the wells. Add antibody against monomethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 μg/ml FLAG peptide

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Yang, C., et al. Cell Res. 2010. 20: 886-898.
2. Osawa, T. et al. Proc. Nat. Acad. Sci. (USA). 2011. 108(51): 20725-20729.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Demethylase

Recombinant Protein