Human Methyltransferase-like 3 (METTL3), GenBank Accession No. NM_019852, 2-580 (end) with N-terminal FLAG-tag, expressed by baculovirus in Sf9 insect cells. MW = 66 kDa.

Human Methyltransferase-like 3, METTL3, RNA

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, 100 µg/ml flag peptide

Aqueous buffer solution.

At least 6 months at –80°C.

Useful as a positive control for Western blot studies.

1. Liu J. et al. Nat. Chem. Biol. 2014. 10: 93–95

2. Wang X. et al. Nature. 2016. 534:575578 

Avoid freeze/thaw cycles. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.

Methyltransferase Enzyme

Protein